Record Display for the EPA National Library Catalog


Main Title Mutagenic and Clastogenic Properties of 3-Chloro-4-(Dichloromethyl)-5-Hydroxy-2(5H)-Furanone: A Potent Bacterial Mutagen in Drinking Water.
Author Meier, J. R. ; Blazak, W. F. ; Knohl, R. B. ;
CORP Author Health Effects Research Lab., Cincinnati, OH. Toxicology and Microbiology Div. ;SRI International, Menlo Park, CA.
Year Published 1987
Report Number EPA/600/J-87/340;
Stock Number PB88-202114
Additional Subjects Furans ; Chromosomes ; Potable water ; Mutagens ; Pharmacology ; Water pollution ; Salmonella typhimurium ; Cell nucleus ; Hamsters ; Mice ; Rats ; Ovary ; Ribosomes ; Toxicity ; Bone marrow ; Drugs ; Contaminants ; Reprints ; Drinking water ; Chromosome aberrations ; Microsomes(Liver) ; Water pollutants(Chemical) ; Cricetulus ; Fibroblasts ; Pollutants
Library Call Number Additional Info Location Last
NTIS  PB88-202114 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 16p
3-Chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) was found to be a direct-acting mutagen in the Ames test for strains TA1535, TA1538, TA92, TA97, TA98, TA100 and TA102. The highest mutagenic response (approximately 13,000 revertants/nmol) was seen in strain TA100. The TA100 response was six- to tenfold higher than in TA98, TA97, and TA102, and 100- to 500-fold higher than in TA1535, TA92, and TA1538. The addition of a 9,000 x g supernatant fraction (S-9) from livers of polychlorinated biphenyl-treated rats, along with cofactors for NADPH generation, resulted in a 90% reduction in the TA100 mutagenicity. MX induced chromosomal aberrations in Chinese hamster ovary cells after 6-8 hr exposure without S-9 at a dose as low as 4 micro/ml, and after 2 hr exposure with S-9 at a dose of 75 micro/ml. The oral dose of MX lethal to 50% (LD50) in Swiss-Webster mice was determined to be 128 mg/kg. MX did not induce micronuclei in mouse bone marrow when administered by oral gavage at doses up to 70% of the LD50. (Copyright (c) 1987 Alan R. Liss, Inc.)