Record Display for the EPA National Library Catalog


Main Title Biochemical Effects of Three Carcinogenic Chlorinated Methances in Rat Liver.
Author Kitchin, K. T. ; Brown, J. L. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/397;
Stock Number PB90-245978
Additional Subjects Toxicology ; Biochemistry ; Liver ; Carbon tetrachloride ; Chloroform ; Chloromethanes ; Glutathione ; Liver neoplasms ; Lethal dosage ; Reprints ; Carcinogenicity tests ; Cell survival ; DNA damage ; Cytochrome P-450 ; Ornithine decarboxylase ; Alanine aminotransferase ; Mutagenicity tests
Library Call Number Additional Info Location Last
NTIS  PB90-245978 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
Three chlorinated methanes, carbon tetrachloride, chloroform and methylene chloride known to cause liver tumors in rodents, were given by oral gavage to adult female rats both 21 and 4 hours before sacrifice. Then hepatic DNA damage, ornithine decarboxylase, cytochrome P-450, glutathione content and serum alanine aminotransferase activity assays were performed. Carbon tetrachloride increased rat hepatic ornithine decarboxylase activity and decreased cytochrome P-450 content at doses both below and above cytotoxicity (as measured by increased serum alanine aminotransferase activity). At 54 and 160 mg/kg, chloroform increased hepatic ornithine decarboxylase activity with minimal or no elevation in serum alanine aminotransferase activity. After oral administration of 480 mg/kg of chloroform, hepatic ornithine decarboxylase activity, serum alanine aminotransferase activity and hepatic DNA damage were increased while hepatic cytochrome P-450 content was decreased. The results with methylene chloride varied between replicates done on three different days. (Copyright (c) 1989 Alan R. Liss, Inc.)