Record Display for the EPA National Library Catalog


Main Title Effects of Methanol on Embryonic Mouse Palate in Serum-Free Organ Culture.
Author Abbott, B. D. ; Logsdon, T. R. ; Wilke, T. S. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Developmental Toxicology Div. ;ManTech Environmental Technology, Inc., Research Triangle Park, NC.
Publisher c1994
Year Published 1994
Report Number EPA/600/J-94/323;
Stock Number PB94-197134
Additional Subjects Teratogenic compounds ; Methanol ; Organ culture ; Cleft palate ; Ethanol ; Embryo ; Serum-free culture media ; Growth ; Morphology ; Proteins ; Deoxyribonucleic acids Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB94-197134 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 16p
The present study examines the developmental toxicity of methanol in the palate using a serum-free organ culture model. Gestation day 12 CD-1 mouse embryos were dissected and mid-craniofacial tissues were cultured in BGJ medium at 37 C for 4 days with medium changes at 24 hr intervals. Cultures were exposed to methanol from 0-20 mg/ml for 6 hr, 12 hr, 1 or 4 days. Some cultures were exposed to ethanol for 4 days at doses ranging from 0-15 mg/ml. All cultures were gassed with a 50% O2, 5% CO2, and 45% N2 upon addition of fresh medium and prior to the addition of alcohol. In general the ethanol was more potent than methanol for inhibition of protein and DNA synthesis and palatal fusion. The study demonstrated that methanol can selectively affect specific sensitive cell populations and has effects on proliferation and cell fate.