||Hydrogenase-Linked Gene in 'Methanobacterium thermoautotrophicum' Strain DeltaH Encodes a Polyferredoxin.
Reeve, J. N. ;
Beckler, G. S. ;
Cram, D. S. ;
Hamilton, P. T. ;
Brown, J. W. ;
||Ohio State Univ., Columbus. Dept. of Microbiology. ;Massachusetts Inst. of Tech., Cambridge. Dept. of Chemistry. ;Harvard Medical School, Boston, MA. Dept. of Biological Chemistry and Molecular Pharmacology.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Methanogenic bacteria ;
Bacterial genes ;
Molecular cloning ;
Open reading frames ;
Molecular sequence data ;
Nucleic acid sequence homology ;
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The genes, mvhDGA which encode the subunit polypeptides of methyl viologen reducing hydrogenase in Methanobacterium thermoautotrophicum strain DeltaH have been cloned and sequenced. These genes, together with a fourth open reading frame designated mvhB, are tightly linked and appear to form an operon which is transcribed starting 42bp upstream of mvhD. The organization and sequences of the mvhG and mvhA genes indicate a common evolutionary ancestry with genes encoding the small and large subunits of hydrogenases in eubacterial species. The product of the mvhB gene is predicted to contain six tandomly-repeated, bacterial ferredoxin-like domains and is therefore predicted to be a poly-ferredoxin which could contain as many as 48 iron atoms in 12 Fe4S4 clusters.