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Main Title Application of Adenylate Energy Charge and Adenine Nucleotide Measurements as Indicators of Stress in 'Nephtys incisa' Treated with Dredged Material.
Author Zaroogian, G. E. ; Johnson., M. ;
CORP Author Environmental Research Lab., Narragansett, RI.
Publisher c1 Feb 89
Year Published 1989
Report Number EPA/600/J-89/484 ;ERLN-747;
Stock Number PB91-117549
Additional Subjects Water pollution ; Physiology ; Toxicology ; Aromatic polycyclic hydrocarbons ; Metals ; Experimental design ; Sediments ; In vivo analysis ; Extraction ; Reprints ; Biological indicators ; Energy metabolism ; Adenine nucleotides ; Dredge spoil ; Biological effects ; Polychlorinated biphenyls ; Nephtys incisa
Library Call Number Additional Info Location Last
NTIS  PB91-117549 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
Initial attempts at measuring AEC in Nephtys incisa yielded low values. A low energy charge most likely indicates a poor extraction of adenine nucleotides in tissue samples from individuals collected from a nonlimiting environment. Thus, there was a need to develop an extraction and handling procedure for N. incisa that consistently produced values for AEC ratios that were representative of in vivo values reported for actively metabolizing cells, (Atkinson 1977). The objective of the study was to evaluate the applicability of AEC as a measure of stress in N. incisa treated with dredged material under laboratory conditions and to determine the degree of variability inherent in the test.