| Main Title |
Induction of 6-Thioguanine Resistance in Synthronized Human Fibroblast Cells Treated with Methyl Methanesulfonate, N-Acetoxy-2-Acethylaminofluorene and N-Methyl-N'-Nitro-N-Nitrosoguanidine. |
| Author |
Huang, Shiu L. ;
Huang, Shu-Mei S. ;
Casperson, Charlotte ;
Waters, Michael D. ;
|
| CORP Author |
Northrop Services, Inc., Research Triangle Park, NC. ;National Inst. of Environmental Health Sciences, Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. |
| Year Published |
1981 |
| Report Number |
EPA-600/J-81-181; |
| Stock Number |
PB83-209726 |
| Additional Subjects |
Humans ;
Nitrogen organic compounds ;
Sulfur organic compounds ;
Deoxyribonucleic acids ;
Reprints ;
Thioguanine ;
Fibroblasts ;
Methane sulfonic acid/(methyl-ester) ;
Fluorene/N-acetoxy-acetylamino ;
Guanidine/N-methyl-N-nitro-N-nitroso ;
Chemical treatments
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB83-209726 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
12p |
| Abstract |
Chemical induction of 6-thioguanine resistance was studied in synchronized human fibroblast cells. Cells initially grown in a medium lacking arginine and glutamine for 24 h ceased DNA synthesis and failed to enter the S phase. After introduction of complete medium, the cells progressed to the S phase after 16 h. DNA synthesis peaked 20 h after removal of nutrient stress and declined. Mutations were induced in S-phase cells by methyl methanesulfonate (MMS), N-acetoxy-2-acetylaminofluorene (NA-AAF) and N-methyl-N nitro-N-nitrosoguanidine (MNNG). Chemical treatments resulted in an increase in the absolute number of mutant colonies and in a dose-dependent mutation frequency. |
