||Colchicine Inhibits Elevations in Both Alveolar-Capillary Membrane Permeability and Lavage Surfactant After Exposure of the Rat to Phosgene.
Ghio, A. J. ;
Hatch, G. E. ;
||Health Effects Research Lab., Research Triangle Park, NC. Inhalation Toxicology Div. ;Duke Univ., Durham, NC. Dept. of Medicine.
Pulmonary alveoli ;
Capillary permeability ;
Bronchoalveolar lavage fluid ;
Pulmonary surfactants ;
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The authors tested the hypothesis that colchicine diminishes the neutrophil influx and associated elevations in permeability of the alveolar-capillary membrane but does not affect lavage surfactant accumulation after COCl2 exposure. Rats were treated with either colchicine or saline prior to COCl2 at 0.5 ppm x 60 min. To measure membrane permeability, (125)I-labeled bovine serum albumin was injected via the tail vein immediately and 1 day after exposure. After 2 h, radioactivity of blood and lavage fluid was measured. Lavage surfactant was quantified as phospholipid immediately and 1 day after exposure. Permeability was elevated immediately but returned to normal values 1 day after inhalation. Phospholipid in the lavage fluid showed no immediate change but was increased 1 day after COCl2 inhalation. Colchicine inhibited both the immediate rise in the permeability index and the elevation of phospholipid 1 day after exposure to phosgene. The authors conclude that colchicine inhibited elevations in both alveolar-capillary membrane permeability and lavage surfactant after exposure to phosgene. (Copyright (c) 1992 by Hemisphere Publishing Corporation.)