| Main Title |
Screening methods for toxic substances / |
| Author |
Sikka, Harish Chandra.
|
| CORP Author |
Syracuse Research Corp., N.Y.;Health Effects Research Lab., Research Triangle Park, N.C. Environmental Toxicology Div. |
| Publisher |
U.S. Environmental Protection Agency, Health Effects Research Laboratory, |
| Year Published |
1978 |
| Report Number |
EPA-600/1-78-014; EPA-68-02-1786 |
| Stock Number |
PB-277 017 |
| OCLC Number |
42843379 |
| Subjects |
Toxicity testing ;
Neurotoxic agents ;
Phenytoin--Toxicology ;
DDT (Insecticide)--Toxicology ;
Neurotoxic agents--Screening
|
| Additional Subjects |
Brain ;
Neurology ;
Toxicology ;
DDT ;
Pesticides ;
Synapses ;
Nervous system ;
Nerve cells ;
Membranes ;
Permeability ;
Ions ;
Pathology ;
Detection ;
Toxicity ;
Tests ;
Sublethal dosage ;
Rats ;
Laboratory animals ;
In vitro analysis ;
Toxic substances ;
Synaptosomes ;
Fluorescent probes
|
| Internet Access |
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| EJBD |
EPA 600-1-78-014 |
c.1 |
Headquarters Library/Washington,DC |
06/09/2014 |
| EJED |
EPA 600/1-78/014 |
|
OCSPP Chemical Library/Washington,DC |
01/13/2001 |
| EKBD |
EPA-600/1-78-014 |
|
Research Triangle Park Library/RTP, NC |
02/23/2000 |
| ELBD ARCHIVE |
EPA 600-1-78-014 |
Received from HQ |
AWBERC Library/Cincinnati,OH |
10/04/2023 |
| ELBD |
EPA 600-1-78-014 |
|
AWBERC Library/Cincinnati,OH |
03/29/2021 |
| NTIS |
PB-277 017 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
v, 18 pages ; 28 cm. |
| Abstract |
The present report deals with the application of fluorescent probe technique to the detection of conformational changes in rat brain synaptosomes under the influence of certain neurotoxic chemicals, chlorophenothane (DDT) and diphenylhydantoin, which are expected to produce functional changes in nerve membranes. Pretreatment of the rats with (1) a single dose of DDT capable of producing neurological symptoms and (2) repeated sub-lethal doses of DDT did not alter the fluorescence characteristics of the hydrophobic probe 8-anilino-1-naphthalene sulfonic acid (ANS) bound to the synaptosomes. DDT treatment also showed no effect on native protein fluorescence of synaptosomes. Exposure of synaptosomes to diphenylhydantoint in vitro did not induce conformational changes as measured by ANS and intrinsic fluorescence. |
| Notes |
"EPA-600/1-78-014." EPA contract no. 68021786; EPA project officer: Larry L. Hall. Includes bibliographical references (pages 16-17). |
