| Main Title |
Metabolism of 2-Methylnaphthalene to Isomeric Dihydrodiols by Hepatic Microsomes of Rat and Rainbow Trout. |
| Author |
Breger, R. K. ;
Franklin, R. B. ;
Lech, J. J. ;
|
| CORP Author |
Medical Coll. of Wisconsin, Inc., Milwaukee.;Environmental Research Lab.-Duluth, MN. |
| Year Published |
1981 |
| Report Number |
EPA-R-806878; EPA-600/J-81-649; |
| Stock Number |
PB84-101310 |
| Additional Subjects |
Ribosomes ;
Naphthalene compounds ;
Rats ;
Fishes ;
In vitro analysis ;
In vitro analysis ;
Reprints ;
Rainbow trout ;
Naphthalene/methyl ;
Dihydrodiols
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB84-101310 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
9p |
| Abstract |
The metabolism of 2-methylnaphthalene in rats (in vivo and in vitro) and rainbow trout (in vitro) has been investigated. Three isomeric dihydrodiols were formed by microsomal preparations and these were isolated and identified by high-pressure liquid chromatography and gas chromatography/mass spectroscopy. The temperature, microsomal protein content, and incubation time were varied to obtain the optimum conditions for their formation. The conversion of 2-methylnaphthalene to both monohydroxylated compounds and dihydrodiols was reduced by incubation with carbon monoxide, the omission of NADPH, or use of heat-denatured microsomes, implying the involvement of cytochrome(s) P-450-linked mixed-function oxidase activity. |
