Record Display for the EPA National Library Catalog


Main Title Demonstration of Multiple Antigenic Determinants on 'Mycoplasma pneumoniae' Attachment Protein by Monoclonal Antibodies.
Author Hu, P. C. ; Huang, C. H. ; Huang, Y. S. ; Collier, A. M. ; Clyde, W. A. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill.
Year Published 1985
Report Number EPA/600/J-85/435;
Stock Number PB86-224599
Additional Subjects Antigens ; Protein ; Immunology ; Bacteria ; Reprints ; Mycoplasma pneumoniae ; Monoclonal antibodies
Library Call Number Additional Info Location Last
NTIS  PB86-224599 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
Distinct multiple antigenic determinants of the attachment protein of Mycoplasma pneumoniae have been identified by limited proteolytic cleavage using specific monoclonal antibodies. Western blots prepared from the gels containing the cleaved fragments were probed with antiserum against M. pneumoniae or monoclonal antibodies. Five distinct bands with intact antigenic determinants were detected by the antiserum of which two bands were each reactable with two monoclonal antibodies. A sequential binding assay suggested that these monoclonal antibodies recognized different antigenic sites of each band. These results demonstrate the existence of multiple antigenic sites on attachment protein and the described procedures should prove useful for identifying those antigenic sites critical to the specific attachment of M. pneumoniae.