| Main Title |
Interim procedures for conducting the Salmonella/microsomal mutagenicity assay (Ames test) / |
| Author |
Williams, Llewellen R. ;
Preston, J. E.
|
| Other Authors |
|
| CORP Author |
Environmental Monitoring Systems Laboratory (Las Vegas, Nev.) |
| Publisher |
U.S. Environmental Protection Agency, Environmental Monitoring Systems Laboratory ; Center for Environmental Research Information [distributor], |
| Year Published |
1983 |
| Report Number |
EPA-600/4-82-068 |
| Stock Number |
PB88-205380 |
| OCLC Number |
10218705 |
| Subjects |
Mutagenicity testing
|
| Additional Subjects |
Mutagenicity testing ;
Mutagens ;
Carcinogens ;
Hazardous materials ;
Bioassay ;
Tests ;
Salmonella ;
In vivo analysis ;
Enzymes ;
Sensitivity ;
Culture media ;
Counting methods ;
Histidine ;
Separation ;
Chemicals ;
Ames test
|
| Internet Access |
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| EHAM |
EPA-600/4-82-068 |
|
Region 1 Library/Boston,MA |
05/25/2016 |
| EJED |
EPA 600/4-82/068 |
|
OCSPP Chemical Library/Washington,DC |
12/17/2004 |
| EKBD |
EPA 600/4-82-068 |
|
Research Triangle Park Library/RTP, NC |
12/04/2018 |
| EKCD |
EPA-600/4-82-068 |
|
CEMM/GEMMD Library/Gulf Breeze,FL |
07/25/2018 |
| ELBD ARCHIVE |
EPA/600-4-82-068 |
Received from HQ |
AWBERC Library/Cincinnati,OH |
10/04/2023 |
| EMBD |
PB88-205380 MF |
|
NRMRL/GWERD Library/Ada,OK |
12/24/1992 |
| ESAD |
EPA 600-4-82-068 |
|
Region 10 Library/Seattle,WA |
03/23/2010 |
| NTIS |
PB88-205380 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
1 volume (various pagings) : 1 form ; 28 cm |
| Abstract |
The Ames assay is a rapid-screening procedure for determining the presence of mutagenic and carcinogenic pure chemicals, complex environmental mixtures, and commercial products. The procedure described incorporates modifications designed to make Ames testing data more precise and reproducible. It is intended as an interim procedure for use in the Agency's hazardous waste monitoring program. The assay uses five standard strains of histidine-requiring Salmonella typhimurium mutants that revert after interaction with mutagens to histidine-independence (his+). Certain mutagens are directly active in the system while others require activation by mammalian enzymes added to the test system. The enzymes perform metabolic conversions mimicking those of mammalian organs in vivo. Test sensitivity to some mutagens is enhanced by additional mutations in the test strains and by the plasmid pKM101 in two of the strains. Counts of revertant colonies on test plates are compared to counts of the same strain on control plates. |
| Notes |
Distributed to depository libraries in microfiche. "Mar. 1983." Includes bibliographical references (pages R-1-R-2). "EPA-600/4-82-068." |
