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RECORD NUMBER: 16 OF 46

OLS Field Name OLS Field Data
Main Title Condensed protocols from Molecular cloning : a laboratory manual
Author Sambrook, Joseph.
Other Authors
Author Title of a Work
Russell, David W.
Sambrook, Joseph.
Publisher Cold Spring Harbor Laboratory Press,
Year Published 2006
OCLC Number 61253855
ISBN 0879697725 (hardcover : alk. paper) 9780879697723 (hardcover : alk. paper) 0879697717 (pbk. : alk. p
Subjects Molecular cloning--Laboratory manuals ; Cloning, Molecular--Laboratory Manuals ; Clonage moleculaire--Manuels de laboratoire
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
EOBM  QH442.2.S24 2006 NEIC Library/Denver,CO 07/18/2008
Collation v, 800 p. : ill. ; 28 cm.
Notes
"A single-volume adaptation of the three-volume third edition of Molecular cloning: a laboratory manual"--P. {4} of cover. Includes bibliographical references and index.
Contents Notes
Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 vectors -- Working with high-capacity vectors -- Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.