Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Sister Chromatid Exchange Analysis in Cultured Primary Lung, Liver, and Kidney Cells of Mice Following In vivo Exposure to Vinyl Carbamate.
Author Campbell, J. A. ; Eppersimons, C. F. ; Kligerman, A. D. ; Petro, A. B. ; Sharief, Y. ;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher cOct 85
Year Published 1985
Report Number EPA/600/J-86/523;
Stock Number PB90-135187
Additional Subjects Lung ; Liver ; Kidney ; Toxicology ; Mice ; Exposure ; In vivo analysis ; Mutagens ; Mitosis ; Reprints ; Sister chromatid exchange ; Vinyl carbonate ; Cultured cells ; Bromodeoxyuridine ; Cell cycle
Library Call Number Additional Info Location Last
NTIS  PB90-135187 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/15/1990
Collation 8p
Methods are described for the short-term culture (48 to 56 h) of lung, liver, and kidney cells from C57B1/6 mice. With these techniques mice can be exposed in vivo to test compounds and the cells grown on cover glasses in the presence of 5-bromo-2'-deoxyuridine (BrdUrd) (5 micro M) for analysis of sister chromatid exchange (SCE) and cell cycle kinetics. Mice exposed to vinyl carbamate (VC) (10 to 60 mg/kg) by ip. injection were used in the initial examination of this system. Cultured lung and kidney cells from exposed animals (60 mg/kg) exhibited significant increases in SCE frequencies (approximately 3 to 5x base-line); however, liver cells were much less responsive and showed less than a twofold increase over baseline SCE levels. Lung cultures initiated as long as 320 h after VC exposure (60 mg/kg) revealed a persistence of lesions leading to the formation of SCEs in vitro. The methodology permits analysis of cytogenetic damage in organs with very low mitotic activity following in vivo exposure to known or suspected genotoxicants. (Copyright (c) 1989 Tissue Culture Association, Inc.)