Record Display for the EPA National Library CatalogRECORD NUMBER: 7 OF 9
|Main Title||In vitro Lymphocyte Proliferation Assays: The Mitogen-Stimulated Response and the Mixed Lymphocyte Reaction in Immunotoxicity Testing.|
|Author||Smialowicz, R. J. ;|
|CORP Author||Health Effects Research Lab., Research Triangle Park, NC.|
|Additional Subjects||Toxicity ; Lymphocytes ; Mitogens ; Cell division ; Immunology ; In vitro analysis ; Lymphocyte transformation ; Test methods ; Spleen ; Rats ; Mice ; Toxic substances ;|
The report describes detailed methodologies for the determination of the in vitro mitogen-stimulated response and one-way mixed lymphocyte reaction of mouse and rat lymphocytes. A list of reagents, supplies and equipment necessary for the successful completion of these assays is provided. Also, a step-by-step description of each of these assays is presented, so that laboratories unfamiliar with these procedures should be able to perform them. These assays are useful for the identification and characterization of agents capable of altering the immune system and as such are useful as screening tests for potential immunotoxicants.