Record Display for the EPA National Library Catalog
RECORD NUMBER: 8 OF 10| Main Title | Hybridization of DNA Probes with Whole-Community Genome for Detection of Genes That Encode Microbial Responses to Pollutants: 'mer' Genes and Hg (2+) Resistance. | |||||||||||
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| Author | Barkay, T. ; Liebert, C. ; Gillman, M. ; | |||||||||||
| CORP Author | Environmental Research Lab., Gulf Breeze, FL. | |||||||||||
| Publisher | c1989 | |||||||||||
| Year Published | 1989 | |||||||||||
| Report Number | EPA/600/J-89/167 ;CONTRIB-664; | |||||||||||
| Stock Number | PB90-129529 | |||||||||||
| Additional Subjects | Toxicity ; Aquatic microbiology ; Genetics ; Mercury ; Reprints ; Microbial sensitivity tests ; DNA probes ; Genomic library ; Nucleic acid hybridization | |||||||||||
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| Collation | 6p | |||||||||||
| Abstract | Nucleic acids extracted from microbial biomass were hybridized with probes representing four mer operons, to detect genes encoding adaptation to Hg2+. An enrichment in sequences similar to the mer genes of transposon 501 occurred during adaptation in a freshwater community. In an estuarine community, all four mer genes were only slightly enriched, suggesting that additional, yet uncharacterized, mer genes encodeded adaptation to HG2+. |