Record Display for the EPA National Library Catalog


Main Title Survival and Effects of Wild-Type, Mutant, and Recombinant 'Streptomyces' in a Soil Ecosystem.
Author Wang, Z. ; Crawford, D. L. ; Pometto, A. L. ; Rafii., F. ;
CORP Author Idaho Univ., Moscow. Dept. of Bacteriology and Biochemistry. ;Iowa State Univ., Ames. ;Idaho Agricultural Experiment Station, Moscow.;Corvallis Environmental Research Lab., OR.
Publisher c1989
Year Published 1989
Report Number EPA-R-813486; EPA/600/J-89/541;
Stock Number PB91-231258
Additional Subjects Terrestrial ecosystems ; Soil microbiology ; Streptomyces ; Mutation ; Genetic recombination ; Survival analysis ; Plasmids ; Genetic engineering ; Lignin ; Microbial colony count ; Minerals ; Soil chemistry ; Carbon dioxide ; Bacterial spores ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB91-231258 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
In a laboratory simulation, selected wild-type, mutant, and recombinant Streptomyces were released into a silt loam soil. Strains included genetically enhanced lignin decomposers and those expressing recombinant plasmids. Their survival and effects on soil organic carbon mineralization were monitored in sterile and non-sterile soil, with and without lignocellulose supplementation. Survival was followed by viable plate counts on selective media. This is the first report of released, genetically altered Streptomyces having a measurable effect on a natural ecosystem.