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Main Title Determination of Plasmid DNA Concentration Maintained by Nonculturable 'Escherichia coli' in Marine Microcosms.
Author Byrd, J. J. ; Leahy, J. G. ; Colwell, R. R. ;
CORP Author Center of Marine Biotechnology, Baltimore, MD.;National Science Foundation, Washington, DC.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1992
Year Published 1992
Report Number NSF-BSR-8806509; EPA/600/J-92/386;
Stock Number PB93-121176
Additional Subjects Aquatic microbiology ; Escherichia coli ; Plasmids ; Deoxyribonucleic acids ; Marine biology ; Cell survival ; Molecular cloning ; Sea water ; Microbial colony count ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB93-121176 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
The concentration of plasmid pBR322 DNA in nonculturable Escherichia coli JM83 was measured to determine whether the plasmid concentration changed during survival of E. coli in marine and estuarine water. E. coli JM83 containing the plasmid pBR322 was placed in both sterile seawater and sterile estuarine water and analyzed for survival (i.e., culturability) and plasmid maintenance. The concentration of pBR322 DNA remained stable in E. coli JM83 for 28 days in an artificial seawater microcosm, even though nonculturability was achieved within 7 days. E. coli JM83 incubated in sterile natural seawater or sterile estuarine water did not reach nonculturability within 30 days. Under all three conditions, plasmid pBR322 DNA was maintained at approximately the initial concentration. Cloning of DNA into the plasmid pUC8 did not alter the ability of E. coli to maintain vector plasmid DNA, even when the culture was in the nonculturable state, but the concentration of plasmid DNA decreased with time in the microcosm. The authors conclude that E. coli is able to maintain plasmid DNA while in the nonculturable state and that the concentration at which the plasmid is maintained appears to be dependent upon the copy number of the plasmid and/or the presence of foreign DNA. (Copyright (c) 1992, American Society for Microbiology.)