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Main Title Generation of cDNA libraries : methods and protocols /
Other Authors
Author Title of a Work
Ying, Shao-Yao.
Publisher Humana Press,
Year Published 2003
OCLC Number 51178265
ISBN 1588290662; 9781588290663; 1592593593; 9781592593590
Subjects Antisense DNA--Laboratory manuals ; DNA, Antisense--https://idnlmnihgov/mesh/D016373 ; Combinatorial Chemistry Techniques--methods--https://idnlmnihgov/mesh/D020650Q000379 ; Gene Library--https://idnlmnihgov/mesh/D015723 ; Kloneren ; Genetische manipulatie ; Wetenschappelijke technieken ; Genbibliothek--(DE-588)4236160-6 ; Methode--(DE-588)4038971-6 ; cDNS--(DE-588)4212297-1 ; Labortechnik ; DNA, Antisense--Laboratory Manuals ; Combinatorial Chemistry Techniques--methods--Laboratory Manuals ; Gene Library--Laboratory Manuals
Internet Access
Description Access URL
Publisher description
Library Call Number Additional Info Location Last
ESBM  QP624.5.A57G46 2003 CPHEA/PESD Library/Corvallis,OR 12/12/2003
Collation xiii, 335 pages : illustrations ; 24 cm.
Includes bibliographical references and index.
Contents Notes
Complementary DNA libraries: an overview / Shao-Yao Ying -- Rapid amplification of cDNA ends / Yue Zhang -- cDNA generation on paramagnetic beads / Zhaohui Wang and Michael G.K. Jones -- Construction of a normalized cDNA library by mRNA-cDNA hybridization and subtraction / Ye-Guang Chen -- Amplification of cDNA ends using PCR suppression effect and step-out PCR / Mikhail V. Matz [and others] -- Use of inverse PCR to clone cDNA ends / Sheng-He Huang, Steven H.M. Chen, and Ambrose Y. Jong -- Construction of size-fractionated cDNA library assisted by an in vitro recombination reaction / Osamu Ohara -- Construction of a full-length enriched and 5'-end enriched cDNA library using the oligo-capping method / Yutaka Suzuki and Sumio Sugano -- cDNA library construction using in vitro transcriptional amplification / Shi-Lung Lin and Henry Ji -- Amplification of representative cDNA pools from microscopic amounts of animal tissue / Mikhail V. Matz -- Single cell cDNA library construction using cycling aRNA amplification / Shi-Lung Lin -- mRNA/cDNA library construction using RNA-polymerase cycling reaction / Shi-Lung Lin and Shao-Yao Ying -- Quality assessment of cDNA libraries / Hans-Jèurgen Fèulle -- Assessment of the quality of mRNA libraries by agarose gel electrophoresis / Tsen-Yin Lin and Shao-Yao Ying -- PACS RT-PCR: a method for the generation and measurement of any poly(A)-containing mRNA not affected by contaminating genomic DNA / Igor Nepluev and Rodney J. Folz -- Single-cell mRNA library analysis by northern blot hybridization / Shi-Lung Lin -- Generation of cDNA libraries for profiling gene expression of given tissues or cells / Xin Zhang, Qiu-Hua Huang, and Ze-Guang Han -- Screening poly (dA/dT( -- )) cDNA for gene identification / San Ming Wang [and others] -- Generation of longer cDNA fragments from SAGE tags for gene identification / Jian-Jun Chen [and others] -- Generation of full-length cDNA libraries enriched for differentiatlly expressed genes / Bakhyt Zhumabayeva [and others] -- Subtractive hybridization for the identification of differentially expressed genes using uracil-DNA glycosylase and mung-bean nuclease / Tsen-Yin Lin and Shao-Yao Ying -- Subtractive cloning of differential genes using RNA-PCR / Shao-Yao Ying and Shi-Lung Lin -- Strategy for construction of a cDNA encoding a repetitive amino acid sequence / Masahiro Asada and Toru Imamura -- Preparing lambda libraries for expression of proteins in prokaryotes or eukaryotes / Rebecca L. Mullinax and Joseph A. Sorge -- Peptide library construction from RNA-PCR-derived RNAs / Shi-Lung Lin -- Identifying interacting proteins in an Escherichia coli-based two-hybrid system / Bonnie Wu, Rebecca L. Mullinax, and Joseph A. Sorge -- Future perspectives / Shao-Yao Ying.