||Individual and Combined Cytotoxic Effects of Cadmium, Copper, and Nickel on Brown Cells of 'Mercenaria mercenaria'.
Zaroogian, G. ;
Anderson, S. ;
Voyer, R. A. ;
||Environmental Research Lab., Narragansett, RI.
Water pollution effects(Animals) ;
Cell survival ;
Divalent cations ;
In vitro analysis ;
Mercenaria Mercenaria ;
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An assay based on the lysosomal incorporation of neutral red dye by brown cells of Mercenaria mercenaria (Bivalvia) was used to measure the cytotoxicity of Cd(sup 2+), Cu(sup 2+), and Ni(sup 2+) singly and in combination. Cytotoxicity was a linear function of Cd(sup 2+) concentration 0.1-1.5 mM and Cu(sup 2+) concentration between 10 and 100 microM. Nickel was not cytotoxic at concentrations as high as 10 mM. The presence of Cu(sup 2+) lessened the cytotoxic effect of Cd(sup 2+). Ni(sup 2+) did not affect cytotoxicity in combination with either Cu(sup 2+) or Cd(sup 2+). Ni(sup 2+) inflated estimates of cell survival by the neutral red assay in this study. Cells exposed to Ni(sup 2+) yielded measured quantities of neutral red dye in excess of those measured in cells from control treatments. (Copyright (c) 1992 Academic Press, Inc.)