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Main Title Individual and Combined Cytotoxic Effects of Cadmium, Copper, and Nickel on Brown Cells of 'Mercenaria mercenaria'.
Author Zaroogian, G. ; Anderson, S. ; Voyer, R. A. ;
CORP Author Environmental Research Lab., Narragansett, RI.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-93/308 ;ERLN-1340;
Stock Number PB93-229300
Additional Subjects Water pollution effects(Animals) ; Toxicity ; Cadmium ; Copper ; Nickel ; Cells(Biology) ; Cell survival ; Divalent cations ; In vitro analysis ; Lysosomes ; Reprints ; Mercenaria Mercenaria ; Brown cells
Library Call Number Additional Info Location Last
NTIS  PB93-229300 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
An assay based on the lysosomal incorporation of neutral red dye by brown cells of Mercenaria mercenaria (Bivalvia) was used to measure the cytotoxicity of Cd(sup 2+), Cu(sup 2+), and Ni(sup 2+) singly and in combination. Cytotoxicity was a linear function of Cd(sup 2+) concentration 0.1-1.5 mM and Cu(sup 2+) concentration between 10 and 100 microM. Nickel was not cytotoxic at concentrations as high as 10 mM. The presence of Cu(sup 2+) lessened the cytotoxic effect of Cd(sup 2+). Ni(sup 2+) did not affect cytotoxicity in combination with either Cu(sup 2+) or Cd(sup 2+). Ni(sup 2+) inflated estimates of cell survival by the neutral red assay in this study. Cells exposed to Ni(sup 2+) yielded measured quantities of neutral red dye in excess of those measured in cells from control treatments. (Copyright (c) 1992 Academic Press, Inc.)