| Main Title |
Specificity of Cellular DNA-Binding Sites of Microbial Populations in a Florida Reservoir. |
| Author |
Paul, J. H. ;
Pichard., S. L. ;
|
| CORP Author |
University of South Florida, St. Petersburg. Dept. of Marine Science.;Environmental Research Lab., Gulf Breeze, FL. |
| Publisher |
c1989 |
| Year Published |
1989 |
| Report Number |
EPA/600/J-89/553; |
| Stock Number |
PB92-129618 |
| Additional Subjects |
Aquatic microbiology ;
Deoxyribonucleic acids ;
Cell membrane ;
Reservoirs ;
Substrate specificity ;
Florida ;
Phosphates ;
Fresh water ;
Oligonucleotides ;
Thymidine ;
Thymine ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB92-129618 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
6p |
| Abstract |
Substrate specificity of the DNA binding mechanism(s) of bacteria in a Florida Reservoir was investigated in short and long term uptake studies with radiolabelled DNA and unlabelled competitors. Thymine oligonucleotides ranging in size from 2 bp to 19-24 bp inhibited DNA binding in 20 min incubations by 43 to 77%. Deoxynucleoside monophosphates, thymidine, and thymine had little effect on short term DNA binding, although several of these compounds inhibited the uptake of radiolabel from DNA in 4 h incubations. RNA inhibited both short and long term radiolabelled DNA uptake equally as well as unlabelled DNA. Collectively these results indicate that aquatic bacteria posses a generalized nucleic acid uptake/binding mechanism specific for compounds containing phosphodiester bonds and capable of recognizing oligonucleotides as short as dinucleotides. |
