| Main Title |
(32)P-Postlabeling Analysis of DNA Adducts of Two Nitrated Polycyclic Aromatic Hydrocarbons in Rabbit Tracheal Epithelial Cells. |
| Author |
Gallagher, J. E. ;
Robertson, I. G. C. ;
Jackson, M. A. ;
Dietrich, A. M. ;
Ball, L. M. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering. |
| Year Published |
1987 |
| Report Number |
EPA/600/D-87/240; |
| Stock Number |
PB88-103718 |
| Additional Subjects |
Aromatic polycyclic hydrocarbons ;
Deoxyribonucleic acids ;
Cells(Biology) ;
Epithelium ;
Trachea ;
Rabbits ;
Marking ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB88-103718 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
9p |
| Abstract |
The 1-nitropyrene (1-NPP and 3-nitrofluoranthene (3-NF) adducts have been analyzed by (32)P-postlabeling and with 1-NP have been compared to the total number of adducts estimated from (14)C binding in rabbit trachael epithelial (RTE) DNA samples. One adduct spot, by (32)P-postlabeling, was detected in the DNA of RTE cells treated with (14)C-1-NP. The adduct, at approximately 6 adducts 10-to the 9th power nucleotides, represents a minor component of the binding to RTE DNA being lesser than 1% of the total (14)C-radioactivity associated with the extracted DNA. Treatment of RTE cells with unlabeled 3-NF resulted in two adduct spots at approximately 12 adducts/10 to the 9th power. When the P1 nuclease treatment was used as an alternative method both the 3-NF derived adducts previously detected following the butanol extraction were absent. |
