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Main Title Effects of the Fungicide Methyl-Benzimidazol-2-yl Carbamate (MBC) on Mouse Germ Cells as Determined by Flow Cytometry.
Author Evenson, D. P. ; Janca, F. C. ; Jost, L. K. ;
CORP Author South Dakota State Univ., Brookings. Dept. of Chemistry.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1987
Report Number EPA-R-810991; EPA/600/J-87/123;
Stock Number PB88-144456
Additional Subjects Fungicides ; Physiological effects ; Testicular diseases ; Germ cells ; Morphology ; Mice ; Male genital diseases ; Toxicology ; Reprints ; Spermatogenesis ; Carbamate/methyl-benzimidazol-2-yl ; Sperm head ; Chromation
Library Call Number Additional Info Location Last
NTIS  PB88-144456 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 15p
Dual-parameter (DNA, RNA) flow cytometry (FCM) measurements were made on testicular and epidiymal sperm cells isolated from mice exposed by oral gavage to 0, 250, 500, or 1000 mg/kg x 5 d of the fungicide methylbezimidazol-2-yl carbamate (MBC), which is known to bind with tubulin subunits and inhibit polymerization and microtubule formation. Effects of exposure to MBC were measured at 7, 24, and 39 d posttreatment. MBC had no effect on body weights, but testis weights and sperm parameters were altered, with few exceptions, only at the highest exposure level. Testis weights were reduced by about 25% at 7 and 24 d after exposure; recovery was observed by 39 d after treatment. FCM measurements of testicular cells showed relative percentages of certain testicular populations (round, elongating, and elongated spermatids) were different from the control pattern 7 and 24 d after treatment. These data demonstrate that spermatogenesis is sensitive to high-dose MBC exposure resulting in an altered ratio of testicular cell types present, abnormal sperm head morphology, and an altered sperm chromatin structure. (Copyright (c) 1987 by Hemisphere Publishing Corporation.)