| Contents Notes |
Detection and quantification of serum or plasma HCV RNA: Mini review of commercially available assays / H. Le Guillou-Guillemette and F. Lunel-Fabiani -- Simultaneous detection of anti-HCV antibody and HCV core antigen / F. Ansaldi and G. Icardi -- Immunohistochemical detection of HCV proteins in liver tissue / O. Galy [and others] -- Nomenclature and numbering of the hepatitis C virus / C. Kuiken and P. Simmonds -- A real-time taqman method for hepatitis C virus genotyping and methods for further subtyping of isolates / K.J. Rolfe [and others] -- Characterization of HCV quasispecies in the hypervariable region 1 (HVR1) by in vitro translation and mass spectrometry / C. Yea, M. Ayers and R. Tellier -- Purification and crystallization of NS5A domain I of hepatitis C virus / J. Marcotrigiano and T. Tellinghuisen -- NS5A phosphorylation and hyperphosphorylation / P. Neddermann -- Preparation and handling of hepatitis C viral proteins NS3 and NS5B for structural studies / S.D. Marco and A. Carfi -- Structural and functional analysis of the HCV p7 protein / N. Saint [and others] -- HCV replicons: overview and basic protocols / V. Lohmann -- Reverse transcription PCR-based sequence analysis of hepatitis C Virus replicon RNA / T.L. Tellinghuisen and B.D. Lindenbach -- Studying HCV RNA synthesis in vitro with replication complexes / W. Yang and M. Huang -- Proteomics study of the hepatitis C virus replication complex / K. Chang, T. Wang and G. Luo -- Investigation of the hepatitis C virus replication complex / V. Brass, R. Gosert and D. Moradpour -- Regulation of interferon regulatory factor 3-dependent innate immunity by the HCV NS3/4A protease / K. Li -- Selection and characterization of drug-resistant HCV replicons in vitro with a flow cytometry-based assay / J.M. Robotham, H.B. Nelson and H. Tang -- High-throughput screening of HCV RNA replication inhibitors by means of a reporter replicon system / W. Hao and R. Duggal -- Inhibition of HCV replication by small interfering RNA / R.B. Ray and T. Kanda -- Cellular receptors and HCV entry / M. Flint and D.M. Tscherne -- Studying HCV cell entry with HCV pseudoparticles (HCVpp) / B. Bartosch and F.L. Cosset -- Screening of small-molecule compounds as inhibitors of HCV entry / J.P. Yang, D. Zhou and F. Wong-Staal -- Isolation of JFH-1 strain and development of an HCV infection system / T. Wakita -- Measuring HCV infectivity produced in cell culture and in vivo / B.D. Lindenbach -- Genotype 1a HCV (H77S) infection system / M. Yi and S.M. Lemon -- Full-length infectious HCV chimeras / T. Pietschmann -- Adaptation of the hepatitis C virus to cell culture / A. Kaul, I. Worz and R. Bartenschlager -- Primary human hepatocyte culture for HCV study / H. Zhu [and others] -- In vivo study of HCV in mice with chimeric human livers / N.M. Kneteman and C. Toso -- Determination of HCV-specific T-cell activity / J.C. Yoon and B. Rehermann -- Analysis of HCV-specific T cells by flow cytometry / M. Shiina and B. Rehermann -- Detection of neutralizing antibodies with HCV pseudoparticles (HCVpp) / M. Dreux and F.L. Cosset -- Mathematical modeling of HCV infection and treatment / H. Dahari [and others]. With the development of robust cell-culturing systems capable of Hepatitis C Virus replication and infection, the past decade has witnessed an explosion of innovative research directed at understanding HCV biology in the context of the authentic replicative cycle of the virus. By providing a compilation of cutting-edge research techniques currently in the use in HCV labs worldwide, Hepatitis C: Methods and Protocols, Second Edition fully updates the previous edition to reflect this progress which promises to unlock the remaining vital information regarding this human pathogen and its interactions. Written in the highly successful Methods in Molecular Biology" series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Fully updated and comprehensives, Hepatitis C: Methods and Protocols, Second Edition serves as a valuable reference guide for basic and clinical researchers using HCV as a model and striving to better understand this critical disease. |
