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Main Title Influence of Cytochrome P450 Mixed-Function Oxidase Induction on the Acute Toxicity to Rainbow Trout 'Salmo gairdner' of Primary Aromatic Amines.
Author Hermens, J. L. M. ; Bradbury, S. P. ; Broderius, S. J. ;
CORP Author Environmental Research Lab.-Duluth, MN. ;Utrecht Rijksuniversiteit (Netherlands). Research Inst. of Toxicology.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/391;
Stock Number PB91-171470
Additional Subjects Mixed function oxidases ; Trout ; Anilines ; Enzyme induction ; Polychlorobiphenyl compounds ; Benzopyrene ; Dose-response relationships ; Liver microsomes ; Metabolic activation ; Water pollution effects(Animals) ; Reprints ; Cytochrome P-450
Library Call Number Additional Info Location Last
NTIS  PB91-171470 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
The influence of enzyme induction on the acute toxicity of aniline and 4-chloroaniline to rainbow trout (Salmo gairdneri) was investigated. For these two xenobiotics, bioactivation reactions are known to occur in mammals. Induction of cytochrome P450 mixed-function oxidase was obtained by intraperitoneal (ip) injection of trout with a mixture of polychlorinated biphenyls (Aroclor 1254). Five days after ip injection with three different doses of Aroclor 1254 (50, 100, and 200 mg/kg), benzo(a)pyrene hydroxylase activity in trout liver microsomes increased five- to sixfold. Cytochrome P450 concentrations in the microsomes were slightly, but significantly, enhanced in two of the three dose levels. The 96-hr LC50's of aniline and 4-chloroaniline were not affected by pretreatment with Aroclor 1254, suggesting that metabolic activation does not necessarily play a role in the acute toxicity of aromatic amines to fish. (Copyright (c) 1990 Academic Press, Inc.)