The Chatanika River and Goldstream Creek, two subarctic streams which lie in roughly parallel valleys in interior Alaska, but that differ in size of watershed, elevation, geomorphology and vegetation were studied over an eighteen month period. Both streams are currently free of human manipulation. Various chemical and physical parameters were measured approximately monthly for fourteen months at nine stations on the Chatanika River and five stations along Goldstream Creek. The occurrences of specific macroinvertebrates in each stream and of the species diversity of each sample are presented. The dissolved oxygen regime of these two streams was also studied.