Record Display for the EPA National Library Catalog


Main Title Flow Cytometric Analysis of the Mechanism of Methylmercury Cytotoxicity.
Author Zucker, R. M. ; Elstein, K. H. ; Easterling, R. E. ; Massaro, E. J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Developmental Toxicology Div. ;NSI Technology Services Corp., Research Triangle Park, NC.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/353;
Stock Number PB91-163675
Additional Subjects Methylmercury compounds ; Toxicity ; Fluorescence spectrometry ; Cell survival ; Flow cytometry ; Dose-response relationships ; Cell membrane ; Erythro leukemia ; Culture tumor cells ; Cell cycle ; Chromosomes ; Mitosis ; Computerized simulation ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB91-163675 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
The target and degree of methylmercury cytotoxicity are dose-dependent. Following 6 h exposure to 2.5 - 7.5 microMolar, methylmercury (MeHg) progressively inhibits DNA synthesis and induces chromosomal damage in murine erythroleukemic cells. However, following exposure to 10 - 50 microMolar MeHg, the plasma membrane/cytoplasm complex is grossly perturbed, cell cycle progression is blocked, and chromosomes appear in ring formations. These findings, together with those previously observed following organotin exposure, also suggest that severe and nonspecific toxicity may be a common endpoint of exposure to high levels of organometals.