||Acute Toxicity of Lead Particulates on Pulmonary Alveolar Macrophages: Ultrastructural and Microanalytical Studies.
deVries, C. R. ;
Ingram, P. ;
Walker, S. R. ;
Linton, R. W. ;
Gutknecht, W. F. ;
||Duke Univ., Durham, NC. ;Veterans Administration Medical Center, Durham, NC. ;Research Triangle Inst., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill. Dept. of Chemistry.;Health Effects Research Lab., Research Triangle Park, NC.
Lead poisoning ;
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Although it is well established that respiratory uptake of lead-containing particles plays a substantial role in the epidemiology of plumbism, relatively little is known about the role of the pulmonary alveolar macrophage in lead poisoning. An in vitro system was designed to investigate the effects of lead oxide particles of respirable size on the rabbit alveolar macrophage. The studies were concerned with the intracellular solubility of PbO and Pb3O4 and changes in fine structure attributable to lead toxicity. The distribution of phagocytosed lead and its intracellular reprecipitation complexes was established by electron microprobe analysis and secondary ion mass spectroscopy in conjunction with transmission electron microscopy, scanning electron microscopy, scanning transmission electron microscopy, and backscatter imaging. It was found that Pb3O4,PbO and PbO-coated particles were ingested by the rabbit alveolar macrophages and that each of these lead oxide compounds produced similar damage to the fine structure of the cell. (Copyright (c) 1983 by the United States-Canadian Division of the International Academy of Pathology).