| Contents Notes |
"High-throughput assays are part of a paradigm shift, to more cost-effective and reliable methods, in the testing of chemical and drug safety. This book covers methods, data generation, data analysis, and applications in risk assessment for major toxicological assays to help toxicologists apply and understand the benefits and limits of high-throughput testing assays. Each chapter describes method principles and includes detailed information on data generation, data analysis, and applications in risk assessment. The topic is especially timely given Europe's REACH legislation and the US National Research Council"-- "This book describes the high-throughput in vitro methods available for toxicity testing, serving as a guide to scientists working in toxicity evaluation and risk assessment of chemicals and drugs"-- PART I General Aspects: 1. ToxCast: Predicting Toxicity Potential Through High-Throughput Bioactivity Profiling / Keith A. Houck, Ann M. Richard, Richard S. Judson, Matthew T. Martin, David M. Reif, and Imran Shah -- 2. High-Throughput Toxicity Testing in Drug Development: Aims, Strategies, and Novel Trends / Willem G.E.J. Schoonen, Walter M.A. Westerink, Femke M. van de Water, and Horbach G. Jean -- 3. Incorporating Human Dosimetry and Exposure Information with High-Throughput Screening Data in Chemical Toxicity Assessment / Barbara A. Wetmore and Russell S. Thomas -- 4. The Use of Human Embryonic Stem Cells in High-Throughput Toxicity Assays / Xin Huang, Dan-yan Zhu, and Yi-jia Lou -- PART II High-Throughput Assays to Assess Different Cytotoxicity Endpoints: 5. High-Throughput Screening Assays for the Assessment of Cytotoxicity / Andrew L. Niles, Richard A. Moravec, Tracy J. Worzella, Nathan J. Evans, and Terry L. Riss -- 6. High-Throughput Flow Cytometry Analysis of Apoptosis / Francesca de Giorgi and Francois Ichas -- 7. High Content Imaging-Based Screening for Cellular Toxicity Pathways / Bram Herpers and Bob van de Water -- 8. The Keratinosens Assay: A High-Throughput Screening Assay to Assess Chemical Skin Sensitization / Andreas Natsch -- 9. High-Throughput Screening Assays to Assess Chemical Phototoxicity / Satomi Onoue, Yoshiki Seto, and Shizuo Yamada -- PART III High-Throughput Assays to Assess Dna Damage and Carcinogenesis: 10. Ames IITM and Ames Liquid Format Mutagenicity Screening Assays / Kamala Pant -- 11. High-Throughput Bacterial Mutagenicity Testing: VitotoxTM Assay / Luc Verschaeve -- 12 Genotoxicity and Carcinogenicity: Regulatory and Novel Test Methods / Walter M.A. Westerink, Joe C.R. Stevenson, G. Jean Horbach, Femke M. van de Water, Beppy van de Waart, and Willem G.E.J. Schoonen -- 13. High-Throughput Genotoxicity Testing: The Greenscreen Assay / Jorg Blumel and Nadine Krause -- 14. High-Throughput Assays to Quantify the Formation of DNA Strand Breaks / Marâya Moreno-Villanueva and Alexander Burkle -- 15. High-Throughput Versions of the Comet Assay / Irene Witte and Andre Stang -- 16. Automated Soft Agar Colony Formation Assay for the High-Throughput Screening of Malignant Cell Transformation / Pablo Steinberg -- 17. High-Throughput Quantification of Morphologically Transformed Foci in Bhas 42 Cells (v-Ha-ras Transfected BALB/c 3T3) Using Spectrophotometry / Kiyoshi Sasaki, Ayako Sakai, and Noriho Tanaka -- PART IV High-Throughput Assays to Assess Reproductive Toxicity, Cardiotoxicity, and Haematotoxicity: 18. ReProGlo: A New Stem-Cell-Based High-Throughput Assay to Predict the Embryotoxic Potential of Chemicals / Frederik Uibel and Michael Schwarz -- 19. Embryonic Stem Cell Test (EST): Molecular Endpoints Toward High-Throughput Analysis of Chemical Embryotoxic Potential / Peter T. Theunissen, Esther de Jong, Joshua F. Robinson, and Aldert H. Piersma -- 20. Zebrafish Development: High-Throughput Test Systems to Assess Developmental Toxicity / Stephanie Padilla -- 21. Single Cell Imaging Cytometry-Based High-Throughput Analysis of Drug-Induced Cardiotoxicity / Min Jung Kim and Joon Myong Song -- 22. High-Throughput Screening Assays to Evaluate the Cardiotoxic Potential of Drugs / Carl-Fredrik Mandenius and Thomas Meyer -- 23. High-Throughput Screening Assays to Evaluate the Hematotoxic Potential of Drugs / Caroline Haglund, Rolf Larsson, and Martin Hoglund -- PART V High-Throughput Assays to Assess Drug Metabolism and Receptor-Related Toxicity: 24. High-Throughput Enzyme Biocolloid Systems for Drug Metabolism and Genotoxicity Profiling Using LC-MS/MS / James F. Rusling and John Schenkman -- 25. Higher-Throughput Screening Methods to Identify Cytochrome P450 Inhibitors and Inducers: Current Applications and Practice / David M. Stresser and George Zhang -- 26. High-Throughput Yeast-Based Assays to Study Receptor-Mediated Toxicity / Johanna Rajasarkka and Marko Virta -- 27. Evaluating the Peroxisomal Phenotype in High Content Toxicity Profiling / Jonathan Z. Sexton and Kevin P. Williams -- 28. A Panel of Quantitative Calux R Reporter Gene Assays for Reliable High-Throughput Toxicity Screening of Chemicals and Complex Mixtures / Bart van der Burg, Sander van der Linden, Hai-yen Man, Roos Winter, Lydia Jonker, Barbara van Vugt-Lussenburg, and Abraham Brouwer -- 29 DR-Calux R : A High-Throughput Screening Assay for the Detection of Dioxin and Dioxin-Like Compounds in Food and Feed / Barbara van Vugt-Lussenburg, Harrie T. Besselink, Bart van der Burg, and Abraham Brouwer. |
