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Main Title Identification of fecal indicator bacteria isolates from an ice-covered river /
Author Sparrow, Elena B, ; Davenport, Charlotte V. ; Gordon, Ronald C.
Other Authors
Author Title of a Work
Davenport, Charlotte V.,
Gordon, Ronald C.,
CORP Author National Heart, Lung, and Blood Inst., Bethesda, MD.
Publisher Environmental Protection Agency, Office of Research and Development [Office of Air, Land, and Water Use], Corvallis Environmental Research Laboratory ;
Year Published 1979
Report Number EPA-600/3-79-073
Stock Number PB81-233017
OCLC Number 05470858
ISBN pbk.
Subjects Water--Microbiology. ; Ice--Microbiology. ; Water--Pollution. ; Water--Bacteriology ; Ice--Bacteriology
Additional Subjects Indicator species ; Bacteria ; Streams ; Coliform bacteria ; Enterobacteriaceae ; Escherichia coli ; Klebsiella ; Streptococcus ; Feces ; Bioindicators ; Water pollution detection
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EHAM  QR105.5.S63 Region 1 Library/Boston,MA 04/29/2016
EJBD ARCHIVE EPA 600-3-79-073 Headquarters Library/Washington,DC 04/10/2014
EJBD  EPA 600-3-79-073 Headquarters Library/Washington,DC 03/22/2016
ELBD  EPA 600-3-79-073 AWBERC Library/Cincinnati,OH 03/06/2018
ESAD  EPA 600-3-79-073 Region 10 Library/Seattle,WA 03/23/2010
NTIS  PB81-233017 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation vi, 13 pages ; 28 cm.
The membrane filter technique was used to enumerate the total coliform (TC), fecal coliform (FC), and fecal streptococcus (FS) populations at seven sample stations on an ice-covered river downstream from a major source of domestic pollution. From each membrane filter population (m-TC, m-FC, and m-FS), 210 typical colonies (30 per station) were selected for verification and biochemical differentiation of the component genera. The 210 m-TC isolates were Klebsiella pneumoniae (46.2%), Escherichia coli (20.5%), Enterobacter sp. (18.6%), other total coliforms (5.2%), and 9.5% which did not verify as total coliforms. Among these m-TC cultures, 114 were verified as fecal coliforms (gas production in EC broth at 44.5C). These 114 fecal coliforms were principally K. pneumoniae (53.5%) and E. coli (35.1%). In contrast, the 210 m-FC cultures were predominantly E. coli (77.6%), with K. pneumoniae (10.0%), other fecal coliforms (3.8%), and those not verified as fecal coliforms (8.6%). Of the 210 m-FS isolates, 167 were identified as enterococci, with 165 being Streptococcus faecalis biotypes. The results suggested the majority of these indicator bacteria originated from warm-blooded animal feces. Also, within each population, no overall differences in low temperature survival of the component genera were noted. However, the evidence does suggest that generic selectivity of the m-TC and m-FC techniques biases determination of the FC population composition, and that the m-FC technique underestimates the FC population density.
"July 1979." Includes bibliographical references.