| Main Title |
Disruption of the 'Saccharomyces cerevisiae' Gene for NADPH-Cytochrome P450 Reductase Causes Increased Sensitivity to Ketoconazole. |
| Author |
Sutter, T. R. ;
Loper, J. C. ;
|
| CORP Author |
Cincinnati Univ., OH. Coll. of Medicine.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. |
| Publisher |
c1989 |
| Year Published |
1989 |
| Report Number |
EPA-CR-813366; EPA/600/J-93/305; |
| Stock Number |
PB93-229276 |
| Additional Subjects |
Fungal genes ;
NADH dehydrogenase ;
Saccharomyces cerevisiae ;
Ketoconazole ;
Microbial sensitivity tests ;
Plasmids ;
Genetic complementation tests ;
Southern blotting ;
Western blotting ;
Reprints ;
Phenotype ;
Candida tropicalis
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-229276 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
12p |
| Abstract |
Strains of Saccharomyces cerevisiae deleted in the NADPH-cytochrome P450 reductase gene by transplacement are 200-fold more sensitive to ketoconazole, an inhibitor of the cytochrome P450 lanosterol 14alpha-demethylase. Resistance is restored through complementation by the plasmid-borne wild type gene from either S. cerevisiae or Candida tropicalis. Neither Southern hybridization nor Western immunoblot techniques provided evidence for a second NADPH-cytochrome P450 reductase gene, suggesting that an alternate pathway may provide for the functions of this reductase in S. cerevisiae. (Copyright (c) 1989 Academic Press, Inc.) |
