||Keratinocyte Cell-Mediated Mutagenesis Assay: Correlation with In vivo Tumor Studies.
Reiners, Jr., J. J. ;
Yotti, L. P. ;
McKeown, C. K. ;
Nesnow, S. ;
Slaga, T. J. ;
||Health Effects Research Lab., Research Triangle Park, NC. ;Tennessee Univ., Oak Ridge. ;Oak Ridge National Lab., TN.
In vivo analysis ;
Cell mediated analysis
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A murine keratinocyte cell-mediated mutagenesis assay was characterized and examined as an in vitro model system for studying the biotransformation of promutagens/procarcinogens by mouse skin. The assay used living cultured newborn SENCAR keratinocytes for the metabolic activation of promutagens and Chinese hamster lung V-79 fibroblasts for detection of resulting mutagens. Mutations at, or affecting, the hypoxanthineguanine phosphoribosyltransferease locus were scored by resistance to 6-thioguanine. The relative mutagenicities of several polycyclic aromatic hydrocarbons (PAHs) in the cell-mediated assay correlated with the in vivo skin tumorigenicity of the PAHs determined in a two-stage carcinogenesis protocol.