Record Display for the EPA National Library Catalog


Main Title Keratinocyte Cell-Mediated Mutagenesis Assay: Correlation with In vivo Tumor Studies.
Author Reiners, Jr., J. J. ; Yotti, L. P. ; McKeown, C. K. ; Nesnow, S. ; Slaga, T. J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Tennessee Univ., Oak Ridge. ;Oak Ridge National Lab., TN.
Year Published 1983
Report Number EPA/600/J-83/228;
Stock Number PB84-232719
Additional Subjects Toxicology ; In vivo analysis ; Neoplasms ; Reprints ; Mutagenesis ; Keratinocytes ; Cell mediated analysis
Library Call Number Additional Info Location Last
NTIS  PB84-232719 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
A murine keratinocyte cell-mediated mutagenesis assay was characterized and examined as an in vitro model system for studying the biotransformation of promutagens/procarcinogens by mouse skin. The assay used living cultured newborn SENCAR keratinocytes for the metabolic activation of promutagens and Chinese hamster lung V-79 fibroblasts for detection of resulting mutagens. Mutations at, or affecting, the hypoxanthineguanine phosphoribosyltransferease locus were scored by resistance to 6-thioguanine. The relative mutagenicities of several polycyclic aromatic hydrocarbons (PAHs) in the cell-mediated assay correlated with the in vivo skin tumorigenicity of the PAHs determined in a two-stage carcinogenesis protocol.