Record Display for the EPA National Library Catalog


Main Title Physicochemical Interaction of 'Escherichia coli' Cell Envelopes and 'Bacillus subtilis' Cell Walls with Two Clays and Ability of the Composite to Immobilize Heavy Metals from Solution.
Author Walker, S. G. ; Flemming, C. A. ; Ferris, F. G. ; Beveridge, T. J. ; Bailey, G. W. ;
CORP Author Guelph Univ. (Ontario).;Environmental Research Lab., Athens, GA.
Publisher c1989
Year Published 1989
Report Number EPA-R-813605; EPA/600/J-89/251;
Stock Number PB90-135500
Additional Subjects Adsorption ; Bacillus subtilis ; Escherichia coli ; Clay minerals ; Chemical reactions ; Cell wall ; Kaolinite ; Centrifuging ; Metals ; Cation exchanging ; Binding ; Waste disposal ; X-ray spectroscopy ; Reprints ; Heavy metals ; Smectite
Library Call Number Additional Info Location Last
NTIS  PB90-135500 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
Isolated Escherichia coli K-12 cell envelopes or Bacillus subtilis 168 cell walls were reacted with smectite or kaolinite clay in distilled deionized water (pH 6.0); unbound envelopes or walls were separated by sucrose density gradient centrifugation, and the extent of adsorption was calculated. At saturation, both clays adsorbed approximately 1.0 mg (dry weight) of envelopes or walls per mg (dry weight) of clay. The addition of heavy metals increased the incidence of planar surface orientations, which suggested that multivalent metal cation bridging was coming into play and was of increasing importance. On a dry-weight basis, the envelope-clay and wall-clay mixtures bound 20 to 90% less metal than equal amounts of the individual components did. This reduction in metal-binding capacity indicates that the adsorption of the wall or envelope to clay has masked or neutralized chemically reactive adsorption sites normally available to metal ions. Transmission electron microscopy and energy-dispersive X-ray spectroscopy confirmed these results.