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Main Title Influence of Alternate Electron Acceptors on the Metabolic Fate of Hydroxybenzoate Isomers in Anoxic Aquifer Slurries.
Author Kuhn, E. P. ; Suflita, J. M. ; Rivera, M. D. ; Young, L. Y. ;
CORP Author Oklahoma Univ., Norman. Dept. of Botany and Microbiology. ;New York Univ. Medical Center, NY. Dept. of Microbiology.;Robert S. Kerr Environmental Research Lab., Ada, OK.;National Science Foundation, Washington, DC.
Publisher c1989
Year Published 1989
Report Number NSF-ECE86-05143; EPA/600/J-89/125;
Stock Number PB90-113259
Additional Subjects Biodeterioration ; Electron acceptors ; Aquifers ; Metabolism ; Oxygen ; Liquid chromatography ; Mass spectroscopy ; Reprints ; Hydroxybenzoate ; Aerobic bacteria ; Methanogenic bacteria ; Isomerism
Library Call Number Additional Info Location Last
NTIS  PB90-113259 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
The biodegradation of hydroxybenzoate isomers was investigated with samples obtained from two sites within a shallow anoxic aquifer. The metabolic fates of the substrates were compared in denitrifying, sulfate-reducing, and methanogenic incubations. Under the latter two conditions, phenol was detected as a major intermediate of p-hydroxybenzoate, but no metabolites were initially found with m- or o-hydroxybenzoate. However, benzoate accumulation was noted when metabolic inhibitors were used with these samples. All metabolites were identified by chromatographic mobility, mass spectral profiles, or both. Autoclaved controls were uniformly incapable of transforming the parent substrates. These results suggest that the anaerobic fate of hydroxybenzoate isomers depends on the relative substitution pattern and the prevailing ecological conditions. Furthermore, since these compounds are central metabolites formed during the breakdown of many aromatic chemicals, these findings may help provide guidelines for the reliable extrapolation of metabolic fate information from diverse anaerobic environments. (Copyright (c) 1989, American Society for Microbiology.)