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Main Title Detection of Environmental Viruses in Sludge: Enhancement of Enterovirus Plaque Assay Titers with 5-Iodo-2'-Deoxyuridine and Comparison to Adenovirus and Coliphage Titers (Journal Version).
Author Williams, F. P. ; Hurst, C. J. ;
CORP Author Health Effects Research Lab., Cincinnati, OH. Toxicology and Microbiology Div.
Publisher 1988
Year Published 1988
Report Number EPA/600/J-88/148;
Stock Number PB89-110530
Additional Subjects Enteroviruses ; Sewage sludge ; Adenoviruses ; Coliphage ; Detection ; Iodine organic compounds ; Reprints ; Waste water treatment ; Iododeoxyuridine ; Uridine/deoxy-iodo
Library Call Number Additional Info Location Last
NTIS  PB89-110530 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
Enteroviruses present in the primary sludge of two wastewater treatment plants were quantitated by plaque assay using a continuous African green monkey kidney cell line (BGM). Incubation of BGM monolayers with 5-iodo-2'-deoxyuridine (50 micrograms/ml) for 4 days prior to use enhanced the number of PFU detected in 10 of 10 concentrated sludge samples. Coliphages also present in these samples were detected using as hosts E. coli C and E. coli A-19, an Hfr strain. E. coli C coliphage titers were consistently higher than E. coli A-19 titers, and were 100-1000 times higher than the enterovirus titers obtained with BGM cells. Adenoviruses present in the sludge samples were detected by immunofluorescence assay. Interestingly, those results suggested that adenoviruses were also more numerous than enteroviruses (as detected by either IDU-treated or untreated BGM plaque assay).