Record Display for the EPA National Library CatalogRECORD NUMBER: 3 OF 13
|Main Title||Differences in Arachidonic Acid Metabolism by Human Myelomonocytic Cell Lines.|
|Author||Madden, M. C. ; Becker, S. ; Koren, H. S. ; Friedman, M. ;|
|CORP Author||Environmental Protection Agency, Research Triangle Park, NC. Cell and Molecular Biology Section. ;North Carolina Univ. at Chapel Hill. Center for Environmental Medicine and Lung Biology. ;ABB Environmental Services, Inc., Chapel Hill, NC.|
|Additional Subjects||Arachidonic acids ; Acute myelomonocytic leukemia ; Human ; Cell line ; Interferon type II ; Phagocytes ; Prostaglandin-endoperoxide synthase ; Lipoxygenase ; Cell differentiation ; High pressure liquid chromatography ; FC receptors ; HLA-DR antigens ; Reprints ;|
The production of arachidonic acid metabolites by the HL60, ML3, and U937 human phagocyte cell lines was determined after incubation with interferon-gamma (IFN-gamma, 500 U/ml) or vehicle for 4 days. Cells were prelabeled with tritiated arachidonic acid, ((3)H)AA, for 4 h, and media supernatants were analyzed by high-performance liquid chromatogrpahy. None of the cell lines produced ((3)H)AA metabolites in large amounts during an unstimulated, basal release period (30 or 60 min). In contrast to the HL60 cells, IFN-gamma-differentiated U937 cells formed primarily cyclooxygenase products and undifferentiated and IFN-gamma-differentiated ML3 cells did not form any ((3)H)AA metabolites in response to A23187. These results indicate the need to be careful in selecting a cell line for use in a phagocyte assay system when cyclooxygenase and/or lipoxygenase products could influence the assay results.