Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title A tissue enzyme assay for chlorinated hydrocarbon insecticides.
Author Cutkomp, L. K.
CORP Author Minnesota Univ., St. Paul. Dept. of Entomology, Fisheries and Wildlife.
Publisher For sale by the Supt. of Docs., U.S. Govt. Print. Off.,
Year Published 1974
Report Number EPA-660/2-73-027; EPA-R-801029; EPA-16030-ELZ; W74-10526
Stock Number PB-234 404
OCLC Number 01213615
Subjects Insecticides--Toxicology. ; Insecticides--Physiological effect. ; Organochlorine compounds. ; Enzymes--Analysis. ; Adenosine Triphosphatases--deficiency. ; Hydrocarbons, Chlorinated. ; Insecticides--adverse effects. ; Adenosinetriphosphatase--deficiency. ; Insecticides, Organochlorine--adverse effects
Additional Subjects Chlorohydrocarbons ; Insecticides ; Acaricides ; Lethal dosage ; DDT ; Pesticides ; Assaying ; Enzymes ; Indicator species ; Toxicology ; Fishes ; Brain ; Phosphorus organic compounds ; Carbamates ; Physiological effects ; Water pollution effects(Animals) ; Phosphatase/adenosine-tri
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJBD ARCHIVE EPA 660-2-73-027 Headquarters Library/Washington,DC 01/12/2015
EJBD  EPA 660-2-73-027 Headquarters Library/Washington,DC 01/12/2015
ERAD  EPA 660/2-73-027 Region 9 Library/San Francisco,CA 03/18/2013
NTIS  PB-234 404 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 01/01/1988
Collation vi, 39 pages illustrations 27 cm.
Certain chlorinated hydrocarbon insecticides, especially DDT and closely related chemicals, tested at low concentrations, adversely affect the ATPase enzyme system. DDT inhibited oligomycin-sensitive Mg(2+)ATPase (mitochondrial) both in vitro and in vivo. About 1 micromolar (1 x 10 to the 6th power M) gave 50% inhibition in fish brain and 0.5 parts per billion of DDT in water inhibited about 50% of mitochondrial Mg(2+)ATPase. Na(+)-K(+)ATPase was not inhibited in brain, but was inhibited in vivo in fish gills. Certain discriminating effects were found among chlorinated hydrocarbons, particularly with respect to inhibition of Mg(2+)ATPase, but the ranking of compounds by enzymic effects does not always parallel toxicity values. Organophosphate and carbamate insecticides were ineffective. Further research is needed both in vitro and in vivo to determine how the adverse effects on the enzymes relate to practical interpretations of effects. The abnormally low ATPase activity in chronically treated fish is the first report of an adverse biochemical effect with sublethal doses of DDT.
"Project 16030 ELZ; Program element 1BA027." Includes bibliographical references (pages 37-38).