Record Display for the EPA National Library Catalog


Main Title Effects of Differently Extracted Endotoxins from Escherichia coli on Rainbow Trout (Salmo Gairdneri).
Author O'Lear, Gerard P. ;
CORP Author Providence Coll., R.I. Dept. of Biology.
Year Published 1973
Report Number DI-14-31-0001-3840; OWRR-A-039-RI; 14769,; A-039-RI(1)
Stock Number PB-223 910
Additional Subjects ( Trout ; Escherichia coli) ; ( Water pollution ; Escherichia coli) ; ( Toxins and antitoxins ; Trout) ; Inoculation ; Fishes ; Bacteria ; Experimental data ; Tests ; Extraction ; Toxicity ; Lethal dosage ; Salmo gairdneri ; Endotoxins ; Water pollution effects(Animals) ; Rainbow trout
Library Call Number Additional Info Location Last
NTIS  PB-223 910 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 21p
A laboratory study on the possible lethal effects of Escherichia coli endotoxin on rainbow trout was conducted. The investigation was conducted using endotoxin extracted from Escherichia coli by four different chemical means. The endotoxin was obtained by four mild extraction methods (ethylene-diamine-tetraacetic acid (EDTA) water, trichloracetic acid, and aqueous ether) in order to more closely approximate the endotoxin naturally released by E. coli during its natural growth cycle. The endotoxin preparations were administered to the trout by introperitoneal injections in an aqueous solution. Results have shown that endotoxin extracted using EDTA caused death in the trout at concentrations of approximately 410.88 mg per kg. body weight and 207.16 mg/kg. body weight. Ether extracted endotoxin also was capable of causing death in trout in two to thirteen days at lower concentrations ranging from an average value of 154.62 mg/kg. body weight to 207.23 mg/kg. body weight. Death seems to occur more rapidly with increased endotoxin and with the use of small (younger) trout.