Record Display for the EPA National Library Catalog


Main Title Effect of Inoculation on the Biodegradation of Weathered Prudhoe Bay Crude Oil.
Author Mueller, J. G. ; Resnick, S. M. ; Shelton, M. E. ; Pritchard, P. H. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL. ;Technical Resources, Inc., Gulf Breeze, FL. ;SBP Technologies, Inc., Atlanta, GA.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/384 ;CONTRIB-765;
Stock Number PB93-121168
Additional Subjects Oil pollution ; Land pollution control ; Beaches ; Inoculation ; Biodeterioration ; Biological treatment ; Prince William Sound ; Alaska ; Microorganisms ; Prudhoe Bay ; Environmental transport ; Oil spills ; Water pollution ; Crude oil ; Weathering ; Reprints ; Exxon Valdez ; Cleanup operations
Library Call Number Additional Info Location Last
NTIS  PB93-121168 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
The grounding of the Exxon Valdez on March 24, 1989, resulted in the release of approx. 42 million liters of Prudhoe Bay crude oil into the waters of Prince William Sound, Alaska. Enrichment cultures from oil-contaminated beach material from Prince William Sound, Alaska, generated both a mixed bacterial community of indigenous, oil-degrading marine microorganisms and a pure culture oil-degrader, strain E12V. The mixed and axenic cultures were used in comparative shake flask studies of inoculation on biodegradation of Prudhoe Bay crude oil. Within 12 h following inoculation of homogenized, oiled beach material with the mixed culture, total CO2 production was increased 2-fold relative to a noninoculated control. Moreover, measurements of phenanthrene degradation (as determined by the release of 14CO2 from (9-14C)phenanthrene) showed a 2- or 3-fold greater degradation when inoculated with either strain E12V or with the mixed culture, respectively. However, as medium was replaced by a simulated tidal cycle, the observed stimulation of CO2 production decreased, and the addition of strain E12V had no greater effect on total CO2 production than the addition of inorganic nutrients alone. Chemical analysis of oil recovered after 7 days incubation also suggested that, while these cultures are capable of efficient biodegradation of Prudhoe Bay crude in liquid culture, inoculation of beach material with high numbers of these microorganisms had little effect on the rate and extent of biodegradation of weathered crude oil. Overall, the sustained stimulatory effect was no greater than that observed with the addition of inorganic nutrients alone.