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Main Title Chlorobenzene Bioreactor Demonstration.
Author Miller, D. ; Spain, J. ; Wallace, W. ; Vogel, C. ;
CORP Author Environmental Protection Agency, Washington, DC.
Publisher Mar 97
Year Published 1997
Report Number EPA-RW57935105-01-1; MIPR-N92-16; AL/EQ-1993-0008;
Stock Number AD-A332 757/4
Additional Subjects Ground water ; Reactor operation ; Chlorobenzene ; Measurement ; Removal ; Degradation ; Demonstrations ; Bacteria ; Biochemistry ; Metabolism ; Mixtures ; Oxygen ; Chemical reactors ; Aromatic compounds ; Biodegradation ; Chlorination ; Dissolving ; Microorganisms ; Bench tests ; Bioreactor inoculation ; Bioremediation ; Fixed film ; Chlorinated aromatic compounds ; Pseudomonas strain js150 ; Ex situ
Library Call Number Additional Info Location Last
NTIS  AD-A332 757/4 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 63p
The technical objective of this research was to test whether above-ground, fixed-film bioreactors can biodegrade complex mixtures of chlorinated aromatic compounds in groundwater. Specifically, the project was designed to test the metabolic capabilities of Pseudomonas Strain JS150 in the field. The approach involved a preliminary field study at Robins AFB GA using two above-ground, fixed-film bioreactors, one colonized with Pseudomonas JS150 and one colonized with indigenous groundwater bacteria, to measure degradation of chlorinated aromatic compounds. Independent variables measured included temperature, pH, dissolved oxygen, and fluctuations in microbial populations. Results from the bench-scale column study indicated that the JS150 isolate had the greatest number of desirable qualities and would be the best selection to utilize in a biofilm reactor. Results from the study indicate that both reactors provided substantial chlorobenzene removal (greater than 95%). Data indicate that bioreactor inoculation may be useful for reducing startup time. No degradation intermediates were detected from either the inoculated or uninoculated reactors.