||Interface of a Reverse-Phase High-Performance Liquid Chromatograph with a Diffuse Reflectance Fourier Transform Infrared Spectrometer.
Conroy, C. M. ;
Griffiths, P. R. ;
Duff, P. J. ;
Azarraga, L. V. ;
||California Univ., Riverside. Dept. of Chemistry. ;Ohio Univ., Athens. Dept. of Chemistry.;Environmental Research Lab., Athens, GA.
Infrared spectroscopy ;
Chemical analysis ;
Environmental surveys ;
High performance liquid chromatography ;
Fourier transform spectroscopy
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
An approach to the interface of a reverse-phase high-performance liquid chromatograph and a Fourier transform infrared spectrometer has been developed in which the solutes eluting from the column are continuously extracted into dichloromethane. The application of both flow cell and solvent elimination techniques was explored. Coextracted water and methanol into the CH2Cl2 precluded the use of flow cells. In the solvent elimination interface, the solutes are first extracted into dichloromethane and subsequently concentrated by differential evaporation. The solutes are then deposited on gently heated KC1 powder, and their diffuse reflectance spectra are measured. The interface operates in a continuous fashion without the need for an auxiliary detector. Spectra can be measured at submicrogram sensitivity using this interface. (Copyright (c) 1984 American Chemical Society.)