Record Display for the EPA National Library Catalog

RECORD NUMBER: 41 OF 819

Main Title Ames bioassay of exhaust soluble organic emissions /
Author Haworth, Steve R. ; Haworth, S. R. ; Lawlor, T. E.
Other Authors
Author Title of a Work
Lawlor, Timothy E.
CORP Author Microbiological Associates, Bethesda, MD.;Environmental Protection Agency, Ann Arbor, MI. Emission Control Technology Div.
Publisher Environmental Protection Agency, Office of Mobile Source Air Pollution Control, Emission Control Technology Division,
Year Published 1984
Report Number EPA 460/3-84-003; EPA-68-03-2923
Stock Number PB84-231505
OCLC Number 757544703
Subjects Air--Pollution--Measurement ; Diesel motor exhaust gas
Additional Subjects Bioassay ; Exhaust emissions ; Organic compounds ; Air pollution ; Biological extracts ; Particles ; Microorganisms ; Mutagens ; Toxicity ; Public health ; Salmonella ; Laboratory animals ; Malignant neoplasms ; Solvent extraction ; Gasoline ; Ames test ; Biological indicators ; Diesel engine exhaust ; Cancer ; Soxhlet extraction ; Light duty vehicles
Internet Access
Description Access URL
https://nepis.epa.gov/Exe/ZyPDF.cgi?Dockey=9100Y1RX.PDF
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
ELCD  EPA 460-3-84-003 NVFEL Library/Ann Arbor, MI 10/26/2011
NTIS  PB84-231505 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation iii, 411 p. ; 28 cm.
Abstract
This report summarizes the results obtained from testing 201 extracts of engine exhaust particulates in the Salmonella/mammalian-microsome mutagenicity assay (Ames test) from June 1980 to December 1982. Exhaust particulates collected on filters were subjected to a 24 hr soxhlet extraction with methylene chloride. Each blown down sample was shipped to Microbiological Associates where it was assayed at least twice in the Ames test in two separate experiments conducted over a period of several weeks. Each Ames test was conducted in triplicate at five dose levels using up to five Salmonella typhimurium tester strains, TA98, TA100, TA1535, TA1537 and TA1538, both with and without metabolic activation by Aroclor induced Fischer rat liver microsomes. Very often, when the amount of available test article was limited, only one or two tester strains (TA98, TA100) were used. The resulting mutagenicity data was sent to the Data Management Branch of the EPA's Biometry Division in RTP in North Carolina where it was entered into a computerized data base and was statistically analyzed using the method of Stead, et al (1,2).
Notes
"April 1984." "EPA 460/3-84-003." "Contract No. 68-03-2923"--T.p. "EPA Project Officer: Craig Harvey"--T.p. Includes bibliographical references (p. 38-44).