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Main Title Species and Strain Comparisons of Immunosuppression by 2-Methoxyethanol and 2-Methoxyacetic Acid.
Author Smialowicz, R. J. ; Riddle, M. M. ; Williams, W. C. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Environmental Toxicology Div.
Publisher Aug 94
Year Published 1994
Report Number EPA/600/J-94/514;
Stock Number PB95-148763
Additional Subjects Species diversity ; Toxicology ; Immunosuppressive agents ; Inbred strains rats ; Plaque assay ; Mice ; Dose-response relationships ; Reprints ; 2-Methoxyethanol ; 2-Methoxyacetic acid ; Trinitrophenyl-lipopolysaccharides
Library Call Number Additional Info Location Last
NTIS  PB95-148763 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
2-Methoxyethanol (ME) and its principal metabolite 2-methoxyacetic acid (MAA) have been shown in our laboratory to be immunosuppressive in male Fischer 344 rats. In this study several strains of 12-week-old female rats and mice were used to compare the immunosuppressive activity of equimolar concentrations of ME and MAA on the trinitrophenyl-lipopolysaccharide (TNP-LPS) antibody plaque-forming cell (PFC) response, which we previously demonstrated to be a sensitive end point. Female inbred Lewis, Fischer 344 and Wistar/Furth, and outbred Sprague-Dawley rats were dosed by gavage with either ME or MAA at dosages of 0.33 to 2.64 mmol/kg/day for 10 consecutive days. Female inbred C3H and C57BL/6J, hybrid B6C3F, and outbred CD-1 mice were similarly dosed with equimolar dosages of 0.66 to 5.28 mmol/kg/day ME or MAA. Lewis and Wistar/Furth rats were found to be the most sensitive strains with suppression levels as low as 0.66 mmol/kg/day ME or MAA. While ME and MAA dosing resulted in suppression of the TNP PFC response in all the rat strains tested, such treatment did not suppress the PFC response in any of the mouse strains examined. These results indicate that under the conditions of this study rats, but not mice, are immunosuppressed by ME and MAA exposure, and that the susceptibility to immunosuppression differs among rat strains.