Record Display for the EPA National Library Catalog


Main Title Development of Methods to Measure Virus Inactivation in Fresh Waters.
Author Ward, R. L. ; Winston, P. E. ;
CORP Author James N. Gamble Inst. of Medical Research, Cincinnati, OH.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1985
Report Number EPA/600/J-85/353;
Stock Number PB86-175486
Additional Subjects Fresh water ; Viruses ; Water pollution control ; Enteroviruses ; Measurement ; Reprints ; Inactivation
Library Call Number Additional Info Location Last
NTIS  PB86-175486 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
The study concerns the identification and correction of deficiencies in methods used to measure inactivation rates of enteric viruses seeded into environmental waters. It was found that viable microorganisms in an environmental water sample increased greatly after addition of small amounts of nutrients normally present in the unpurified seed virus preparation. This burst of microbial growth was not observed after seeding the water with purified virus. The use of radioactively-labeled poliovirus revealed that a high percentage of virus particles, sometimes >99%, were lost through adherence to containers, especially on less turbid waters. This effect was partially overcome by the use of polypropylene containers and by the absence of movement during incubation. Adherence to containers clearly demonstrated the need for labeled viruses to monitor losses in this type of study. Loss of viral infectivity in samples found to occur during freezing was avoided by addition of broth.