| Main Title |
Gene Probe for PO Messenger RNA Used to Index Acrylamide Toxic Neuropathy in Rats. |
| Author |
Veronesi, B. ;
Jones, K. ;
Gupta, S. ;
Pringle, J. ;
Mezei., C. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;Dalhousie Univ., Halifax (Nova Scotia). Dept. of Biochemistry. |
| Publisher |
c1991 |
| Year Published |
1991 |
| Report Number |
EPA/600/J-92/023; |
| Stock Number |
PB92-143957 |
| Additional Subjects |
Toxicity ;
Acrylamides ;
Messenger RNA ;
DNA probes ;
Nervous system ;
Immunohistochemistry ;
Myelin proteins ;
Glycoproteins ;
Northern blotting ;
Electron microscopy ;
Genetic transcription ;
Axons ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB92-143957 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
20p |
| Abstract |
Cumulative exposure to the neurotoxicant acrylamide produces axonal damage in the distal ends of both central (CNS) and peripheral (PNS) nerve fibers and subsequent hind-limb paralysis. The messenger RNA which codes for the PNS myelin glycoprotein PO(PO-mRNA) was used to monitor this toxic neuropathy in Sprague Dawley rats prior to, concurrent with, and subsequent to, ultrastructurally and immunocytochemically defined nerve damage. Rats were dosed every other day with acrylamide (50 mg/kg, IP) and sampled intermittently throughout a 4 week exposure period. Slot blot and Northern gel analyses of the proximal and distal sciatic nerve were used to determine a quantitated measure of PO-mRNA. Results suggest that changes in neural specific mRNAs are sensitive to neurotoxic damage and can be used to monitor the pathogenesis of nerve degeneration. |
