Record Display for the EPA National Library Catalog


Main Title Gene Engineering in Yeast for Biodegradation: Immunological Cross-Reactivity among Cytochrome P-450 System Proteins of 'Saccharomyces cerevisiae' and 'Candida tropicalis'.
Author Loper, J. C. ; Chen, C. ; Dey, C. R. ;
CORP Author Cincinnati Univ. Medical Center, OH.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1993
Year Published 1993
Report Number EPA-R-810605; EPA/600/J-93/307;
Stock Number PB93-229292
Additional Subjects Genetic engineering ; Biodeterioration ; Cross reactions ; Cytochrome P-450 ; Saccharomyces cerevisiae ; Hazardous wastes ; Immune sera ; Aromatic polycyclic hydrocarbons ; Polyacrylamide gel electrophoresis ; Reprints ; Candida tropicalis
Library Call Number Additional Info Location Last
NTIS  PB93-229292 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
Yeasts are eukaryotic microorganisms whose cytochrome P-450 monooxygenase systems may be amenable to genetic engineering for the hydroxylation and detoxication of polychlorinated aromatic hydrocarbons. The molecular genetic properties of strains of bakers yeast, Saccharomyces cerevisiae, and an n-alkane utilizing yeast, Candida tropicalis ATCC750 are examined. Standard methods were used to purify cytochrome P-450 and NADPH-cytochrome c (P-450) reductase proteins from cells cultured by semi-anaerobic glucose fermentation (S. cerevisiae, C. tropicalis) and by growth on tetradecane (C. tropicalis). Polyvalent antisera prepared in rabbits to some of these proteins were used in tests of immunological relatedness among the purified proteins using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and nitrocellulose filter immunoblots. The results provide evidence for gene relationships which should prove useful in gene isolation and subsequent engineering of P-450 enzyme systems in yeast.