Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Comparative Effects of Two Polychlorinated Biphenyl Congeners on Calcium Homeostasis in Rat Cerebellar Granule Cells.
Author Kodavanti, P. R. S. ; Shin, D. S. ; Tilson, H. A. ; Harry, G. J. ;
CORP Author National Inst. of Environmental Health Sciences, Research Triangle Park, NC. Div. of Intramural Research.;Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div.
Publisher c1993
Year Published 1993
Report Number EPA-R818550-01-0; EPA/600/J-94/072;
Stock Number PB94-141488
Additional Subjects Cerebellum ; Polychlorobiphenyl compounds ; Calcium ; Homeostasis ; Comparison ; In vitro analysis ; Synaptosomes ; Ca(2+)-transporting ATPase ; Dopamine ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB94-141488 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 05/14/1994
Collation 12p
We compared the effects of two polychlorinated biphenyls (PCB) congeners on Ca(2+) homeostasis in cerebellar granule cells: 2,2'-dichlorobiphenyl (DCBP), a putative neurotoxic congener, and 3,3',4,4',5-pentachlorobiphenyl (PCBP), a presumed nonneurotoxic congener. In cerebellar granule cells (6-8 days in vitro), DCBP was cytotoxic as indicated by a significant increase in LDH leakage at 200 microMols after 2 hr of exposure and at 100 microMols after 4 hr exposure. PCBP, on the other hand, did not affect LDH leakage even at 200 microMols for up to 4 hr. Although both congeners increased cerebellar granule cell (Ca(2+))i, DCPB was more effective in increasing (Ca(2+))i to a greater extent than PCBP. The increase in (Ca(2+))i produced by both congeners was not transient, but a steady rise was observed with time. The results of this study support the hypothesis that the position of chlorine substitution on the biphenyl ring and/or number of chlorine substitutions may have significant implications for predicting potential effects of PCB congeners in the nervous system, and perturbations in Ca(2+) homeostasis might play a significant role in the neuroactivity of PCBs.