Record Display for the EPA National Library CatalogRECORD NUMBER: 10 OF 10
|Main Title||Teratological Effects of 2,4-Dinitrophenol, 'Produced Water' and Naphthalene on Embryos of the Inland Silverside 'Menidia beryllina'.|
|Author||Middaugh, D. P. ; Hemmer, M. J. ; Lores, E. M. ;|
|CORP Author||Environmental Research Lab., Gulf Breeze, FL.|
|Report Number||EPA/600/J-88/107 ;GULF BREEZE CONTRIB-613;|
|Additional Subjects||Marine fishes ; Pesticides ; Toxicity ; Tests ; Embryos ; Herrings ; Trout ; Exposure ; Malformations ; Abnormalities ; Skel ; Concentration(Composition) ; Response ; Phenols ; Cardiovascular system ; Naphthalene ; Crude oil ; Reprints ; Menidia beryllina ; Silversides ; Clupea harengus ; Atlantic herrings ; Teratogens ; Dinitrophenol|
Embryos of the inland silverside, Menidia beryllina, were exposed to three known (or suspected) teratogens: (1) 2,4-dinitrophenol; (2,4-DNP), (2) 'produced water' (PW); and (3) naphthalene (NPH). Tests were conducted by placing single embryos in glass tissue culture tubes containing 6 ml of saline exposure media. Twenty tubes were used for each exposure concentration and controls. A severity-index based upon craniofacial, cardiovascular and skeletal terata was used to rank responses each day. The compounds tested caused teratogenic expressions in embryos and larvae exposed from the 2 to 4 cell and blastula stage through 7 to 8 days post-fertilization.