Record Display for the EPA National Library Catalog


Main Title Use of Carbon Dioxide Challenge to Detect Toxicant - Induced Changes in Cardiopulmonary Function of Awake Rats.
Author Tepper, J. S. ; Wiester, M. J. ; King, M. E. ; Weber, M. F. ; Costa., D. L. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Toxicology Branch. ;Northrop Services, Inc., Research Triangle Park, NC.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/550;
Stock Number PB91-116038
Additional Subjects Toxicology ; Carbon dioxide ; Rats ; Reprints ; Heart function tests ; Respiratory function tests ; Air pollution effects(Animals) ; Computer applications ; Wakefulness ; Hemodynamics
Library Call Number Additional Info Location Last
NTIS  PB91-116038 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 19p
A system has been developed to measure simulatneously the effects of inhaled toxicants on cardiopulmonary function in four awake rats before, during and after exposure. One day prior to testing, Fischer-344 and Sprague-Dawley rats were implanted with an intrapleural or carotid catheter. On the day of testing, the rats were placed into a restraint system to reduce movement artifacts and to keep rats from disturbing the implanted catheters without imposing limitations on functional measurements. The rats were then sealed into constant-volume plethysmographs mounted on the door of a 0.32-cu m Rochester exposure chamber. Thirty minutes after the rats were placed into the plethysmographs, control measurements were obtained. Under the control of a digital computer, the rats were exposed for 15-min periods to 4% CO2. During testing, tidal volume, intrapleural pressure, blood pressure, and the electrocardiogram were recorded on the polygraph, and were digitized and stored in the computer. Four-second breathing epochs were recorded and analyzed, and the results were displayed on line.