Main Title |
Application of DNA-DNA Colony Hybridization to the Detection of Catabolic Genotypes in Environmental Samples. |
Author |
Sayler, G. S. ;
Shields, M. S. ;
Tedford, E. T. ;
Breen, A. ;
Hooper, S. W. ;
|
CORP Author |
Tennessee Univ., Knoxville.;Robert S. Kerr Environmental Research Lab., Ada, OK. |
Year Published |
1985 |
Report Number |
EPA/600/J-85/116; |
Stock Number |
PB86-101839 |
Additional Subjects |
Bacteria ;
Toluene ;
Naphthalene ;
Aromatic hydrocarbons ;
DNA ;
Hybridization ;
Chemical attack(Degradation) ;
Environmental microbiology ;
Molecular cloning ;
Environmental pollutants ;
Plasmids
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB86-101839 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
10p |
Abstract |
The application of preexisting DNA hybridization techniques was investigated for potential in determining populations of specific gene sequences in environmental samples. Cross-hybridizations among two degradative plasmids, TOL and NAH, and two cloning vehicles, pLAFRI and RSF1010, were determined. The detection limits for the TOL plasmid against a nonhomologous plasmid-bearing bacterial background was ascertained. |